Editorial Reference / Tools
Peptide Reconstitution Calculator
Enter what is in the vial, how much bacteriostatic water you added, and your dose. The tool returns the exact volume to draw, in insulin-syringe units, and handles milligrams as well as IU for growth hormone.
Draw to
10 units
= 0.1 mL on a 100-unit syringe
- Concentration
- 2.5 mg/mL
- value
- 2,500 mcg/mL
- Doses per vial
- 20
5 mg in 2 mL = 2.5 mg/mL. For a 250 mcg dose, draw 10 units (0.1 mL) on a 100-unit syringe. 20 doses per vial.
This tool does arithmetic on the numbers you enter. It is educational reference material, not medical advice, a dosing recommendation, or an endorsement of any compound. Many research peptides are prescription-only or investigational and are not approved for human use. Its output is only as accurate as the vial’s actual labelled content, which is why an independently verifiable certificate of analysis matters.
How the calculation works
Reconstitution is one division problem repeated three times. First, concentration: the amount of peptide in the vial divided by the volume of bacteriostatic water you add. A 5 mg vial in 2 mL of water is 2.5 mg/mL, or 2,500 mcg/mL. Adding more water does not change how much peptide you have; it only spreads the same peptide through more liquid, which lowers the concentration and raises the volume you draw for a given dose.
Second, the draw: your dose divided by the concentration gives the volume to pull into the syringe. A 250 mcg dose at 2,500 mcg/mL is 0.1 mL. Third, syringe units: an insulin syringe is marked in units, not millilitres, and on a U-100 syringe 1 mL is 100 units, so 0.1 mL is 10 units. The calculator does all three steps and shows the unit mark to draw to.
Milligrams and IU (growth hormone)
Growth hormone is usually dosed in international units (IU) rather than milligrams. Recombinant human growth hormone (somatropin) is standardised at roughly 3 IU per milligram, so a 10 mg vial holds about 30 IU. The calculator converts between the two using that factor, which you can adjust, so you can enter the vial in milligrams and your dose in IU (or the reverse) and still get the correct draw. HCG is also supplied and dosed in IU and works the same way.
Reading the result
Draw to the unit mark shown. If the volume needed is larger than a single syringe holds, the tool says so; the fix is to reconstitute with more water next time, which lowers the concentration and puts the dose within one syringe. The “doses per vial” figure is the whole number of doses the vial yields at your chosen dose, useful for planning and for sanity-checking that the concentration is sensible.
Two companion references cover the inputs in depth: how the diluent itself affects quality in bacteriostatic water, sterility, and reconstitution, and how a reconstituted peptide holds up over time in peptide stability, storage, and shelf life. The number the calculator returns is only meaningful if the vial actually contains what the label says, which is what a certificate of analysis is for.
Doses reported in studies
The figures below are amounts reported in FDA labels, clinical trials, or peer-reviewed research. They describe what published studies used, and are reference material only, not a recommendation, a protocol, or an endorsement. Many of these compounds are prescription-only or investigational, and several research peptides have no established human dose at all, which is stated plainly here rather than filled with anecdotal numbers. Enter your own figure in the calculator above.
SemaglutideOzempic, Wegovy
FDA label0.25 mg escalating to 2.4 mg SC once weekly (titrated at 4-week steps) (SC)
FDA-approved (type 2 diabetes; chronic weight management) Source
TirzepatideMounjaro, Zepbound
FDA label2.5 mg start, titrated in 2.5 mg steps to a maximum of 15 mg SC once weekly (SC)
FDA-approved (type 2 diabetes; weight management) Source
TesamorelinEgrifta
FDA label1.4 mg SC once daily (1.28 to 2 mg across formulations) (SC)
FDA-approved (HIV-associated lipodystrophy) Source
PT-141 (bremelanotide)Vyleesi
FDA label1.75 mg SC as needed (maximum one dose per 24 h, eight per month) (SC)
FDA-approved (HSDD in premenopausal women) Source
Human Growth Hormone (somatropin)HGH, Genotropin, Norditropin
FDA labelAdult GH deficiency about 0.2 mg/day SC (range 0.15 to 0.3), titrated by IGF-1; roughly 0.45 to 0.9 IU/day (SC)
FDA-approved (multiple brands and indications) Source
HCGPregnyl, Novarel
FDA label500 to 4,000 USP units (IU) IM, indication-dependent (dosed in IU, never mg) (IM)
FDA-approved (hypogonadotropic hypogonadism, others) Source
SermorelinGHRH (1-29), Geref
FDA label30 mcg/kg/day SC (pediatric GH deficiency); about 1 mcg/kg IV as a diagnostic (SC / IV)
Formerly FDA-approved (Geref); withdrawn commercially, now compounded Source
Thymosin Alpha-1Thymalfasin, Zadaxin
Clinical trial1.6 mg SC twice weekly (chronic hepatitis B pivotal trials) (SC)
Approved in 30+ countries, not FDA-approved in the US Source
RetatrutideLY3437943
Clinical trial1 to 12 mg SC once weekly in the Phase 2 obesity trial (2 mg start to limit GI effects) (SC)
Investigational, not approved (Phase 3 TRIUMPH ongoing) Source
Ipamorelin
Clinical trial0.03 mg/kg IV twice daily (Phase 2 postoperative-ileus trial; primary endpoint not met) (IV)
No approved indication; body-composition use is research only Source
CJC-1295 (with DAC)Mod GRF (1-29) is the no-DAC form
Clinical trial1 to 30 mcg/kg SC (Phase 1, long-acting DAC form). The no-DAC form has no comparable human data (SC)
Not FDA-approved; research use only Source
Kisspeptin-54
Clinical trial1.6 to 12.8 nmol/kg SC (IVF oocyte-maturation trigger). Doses are molar, not mass (SC)
Investigational; research use only Source
Melanotan-2MT-II (not bremelanotide)
Clinical trial0.025 mg/kg SC in erectile-function studies (SC)
Not approved anywhere; distinct from FDA-approved bremelanotide Source
GHRP-2 (pralmorelin)
Research literatureAbout 1 mcg/kg IV bolus (100 mcg diagnostic dose approved in Japan) (IV)
Diagnostic approval in Japan only; not FDA-approved Source
GHRP-6
Research literatureAbout 1 mcg/kg IV bolus in acute GH-release studies (IV)
Never approved; research use only Source
HexarelinExamorelin
Research literature0.5 to 2 mcg/kg IV in acute GH-release studies (IV)
Never approved; research use only Source
DSIPDelta sleep-inducing peptide
Research literature25 nmol/kg IV in small 1980s insomnia studies (molar dose) (IV)
Never approved; research use only Source
Selank
Research literatureAbout 600 to 2,700 mcg/day intranasal (registered in Russia). No established injectable dose (Intranasal)
Registered in Russia (intranasal) only; injectable use is research only Source
Semax
Research literature0.1% to 1% intranasal solution (registered in Russia). No established injectable dose (Intranasal)
Registered in Russia (intranasal) only; injectable use is research only Source
BPC-157
No human doseNo established human dose.
No established human dose; Rx-only (Schedule 4) in Australia since June 2024; research use only Source
TB-500thymosin beta-4 fragment
No human doseNo established human dose.
No established human dose. Human trials used IV full-length thymosin beta-4 (450 to 1,200 mg), not this SC fragment Source
GHK-Cucopper tripeptide-1
No human doseNo established human dose.
No established injectable dose (published human data are topical/cosmetic only); research use only Source
MOTS-c
No human doseNo established human dose.
No completed human trial of MOTS-c; no established dose; research use only Source
EpithalonEpitalon
No human doseNo established human dose.
No established dose for the synthetic tetrapeptide (human data are for the pineal extract Epithalamin); research use only Source
NAD+
No human doseNo established human dose.
No FDA-approved injectable product and no controlled trial establishing an injectable dose
Weight-based figures (mcg/kg, mg/kg) and molar figures (nmol/kg) are shown as the studies expressed them; turning them into a fixed dose needs body weight or molecular weight and is outside what this calculator does. Always confirm a compound’s legal status and speak to a qualified clinician. This page is not medical advice.